Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via decellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM was seeded with MenSCs at the density of 3 × 10⁴ cells/cm² and implanted onto a rat's 1.50 × 1.50 cm² full-thickness excisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSC-seeded DAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicated that the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

Effect of saffron on rat sperm chromatin integrity

Currently, relation between reactive oxygen species [ROS] ROS concentration and semen quality was indicated. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. The aim of this study was to evaluate the protection potency of saffron and vitamin E on sperm chromatin integrity. Thirty adult male Wistar rats divided equally into saffron [100 mg/kg], vitamin E [100 mg/kg] and control [0.5cc distilled water /day] groups. After 60 days, cauda epididymis dissected and sperm cells were used for analysis of sperm chromatin packaging by chromomycin A3 [CMA3] staining, and sperm chromatin susceptibility to acid denaturation by acridine orange [AO] staining. The mean percentage of CMA3 positive sperm was significantly decreased in saffron and vitamin E groups relative to control group [p<0.001]. Moreover, the AO staining results showed that the mean percentage of sperm with DNA damage was significantly decreased in saffron and vitamin E groups as compared with control group [p<0.001]. Our results purposed that saffron can protect sperm against DNA damage and chromatin anomalies